Thermo Fisher Scientific KIT Monoclonal Antibody (OTI1B6), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

KIT (c-KIT) is a proto-oncogene and a type III transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). It was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit.

KIT and its ligand regulate the growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are associated with:

• Gastrointestinal stromal tumors (GIST)
• Mast cell disease
• Acute myelogenous leukemia
• Piebaldism

Deregulation of KIT receptor tyrosine kinase (TK), particularly by the D816V mutation, is a therapeutic target in neoplastic mast cell growth.

Expression: Hematopoietic stem cells, germ cells, mast cells, and interstitial cells of Cajal in the gastrointestinal tract.
Upon SCF binding, c-KIT dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues (e.g., Tyr703), enabling docking of Grb2 and activation of the Ras/ERK pathway.
The SCF/c-KIT axis also activates PI3K, PLC-γ, and JAK/STAT signaling pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.