Thermo Fisher Scientific BRRN1 Polyclonal Antibody
Thermo Fisher Scientific의 BRRN1 Polyclonal Antibody는 인간 BRRN1 단백질에 반응하는 고품질 항체입니다. Western blot, ELISA, IP에 적합하며, 0.93 mg/mL 농도의 액상 형태로 제공됩니다. Affinity chromatography로 정제되었으며, -20°C에서 안정적으로 보관 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:8,000–1:35,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to amino acids 435–460 of Human BRRN-1 (NCAPH, UniProt ID: Q15003-1). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity occurs against human BRRN1 protein. BLAST analysis shows 100% homology (18/18) with human and chimpanzee proteins. Partial cross-reactivity may occur with mouse, dog, rat, and chicken based on sequence homology of the amino terminal 13 amino acids.
Core immunogen sequence (A-G-P-D-H-W-R-F) shows 100% identity with numerous proteins from various organisms.
If multiple banding patterns appear in blots, confirm BRRN1-specific reactivity using peptide competition.
Reactivity with BRRN1 proteins from other sources is not known.
Target Information
This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex, which is essential for converting interphase chromatin into condensed chromosomes. The protein associates with mitotic chromosomes except during the early phase of condensation. During interphase, it exhibits distinct punctate nucleolar localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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