Thermo Fisher Scientific G-CSF Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Neutralization: To yield one-half maximal inhibition (ND₅₀) of the biological activity of mG-CSF (0.5 ng/mL), 0.005–0.010 µg/mL of this antibody is required.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

G-CSF (Granulocyte Colony Stimulating Factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described.

This pleiotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells. G-CSF influences proliferation, differentiation, and activation of hematopoietic cells and is expressed by various transformed cells such as carcinoma and myeloblastic leukemia cells.

G-CSF is encoded by two distinct DNA sequences, resulting in a full-size, high-activity and a shorter, low-activity isoform. It is highly conserved among species and functions via interaction with its receptor on hematopoietic progenitors, neutrophilic granulocytes, and certain carcinoma cell lines.

Clinically, G-CSF is used in treatments for neonatal infections, acute myocardial infarction, granulocyte transfusion in neutropenia, severe infections, sepsis, chronic autoimmune neutropenia, acute myeloid leukemia, Sweet’s syndrome, and AIDS. It is also a marker protein for certain carcinomas such as bladder cancer, and dysfunction of G-CSF has been linked to Kostmann Syndrome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.