Thermo Fisher Scientific Goat anti-Rat IgG Fab Secondary Antibody, Biotin
Biotin으로 결합된 염소 유래 anti-Rat IgG Fab 2차 항체로 Western blot, IHC, ELISA 등에 사용 가능. 높은 특이성과 감도를 제공하며, 신호 증폭에 적합. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Rat IgG Fab Secondary Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG whole molecule |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10981278 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling users to use various detection systems such as HRP, AP, or fluorescence.
They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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