Thermo Fisher Scientific CD3e (T-Cell Marker) Monoclonal Antibody (UCHT1)
CD3e 단백질을 표적하는 UCHT1 클론의 Mouse IgG1 단일클론 항체로, 인간 및 침팬지 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 면역분석에 적합하며, 고순도 Protein A/G 정제 및 무보존제 PBS 용액 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| ELISA | 2–4 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chimpanzee, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Immunogen | Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the surface expression of the TCR complex.
T cell activation through the TCR involves the cytoplasmic tails of CD3 gamma, delta, epsilon, and zeta subunits — structurally related members of the immunoglobulin superfamily encoded by genes on human chromosome 11.
CD3 subunits have long cytoplasmic tails that associate with signal transduction molecules, mediated by a double tyrosine-based motif. CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation.
The CD3 antigen is expressed on:
- 68–82% of normal peripheral blood lymphocytes
- 65–85% of thymocytes
- Purkinje cells in the cerebellum
It is not expressed on B or NK cells. Decreased T lymphocyte percentages may occur in some autoimmune diseases.
Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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