Thermo Fisher Scientific GFR alpha-1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 345-420 of human GFRA1 (NP_005255.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GFR alpha-1,uniProtId:P56159-1,ncbiNodeId:9606,antigenRange:345-420,antigenLength:465,antigenImageFileName:PA5-120909_GFR_alpha-1_P56159-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120909_GFR_alpha-1_P56159-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.24 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914481

Product Specific Information

Positive test controls include: 293T, SH-SY5Y, MCF7, Mouse brain, Rat brain. The target is usually found in the following locations: Cell membrane, GPI-anchor, Lipid-anchor.

Immunogen sequence: FGNGSDVTVW QPAFPVQTTT ATTTTALRVK NKPLGPAGSE NEIPTHVLPP CANLQAQKLK SNVSGNTHLC ISNGNY

Target Information

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFR-a-1 recently. GFR-a-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFR-a and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.