
Thermo Fisher Scientific T4 beta-glucosyltransferase
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Thermo Scientific™
T4 beta-glucosyltransferase
Thermo Scientific T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in자세히 알아보기
Thermo Scientific T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating ß-glucosyl-5 hydroxymethylcytosine.
Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1 μg DNA at 37°C in 15 minutes.
Highlights
• Specific—selectively transfers glucose to the hydroxymethyl moiety of 5-hmC
• Fast—complete glucosylation of 1 μg DNA in just 15 min
• Convenient—supplied with optimized buffer and UDP-glucose.  
Applications
• Locus specific detection of 5-hmC
• Enrichment of 5-hmC containing DNA
• Labeling of 5-hmC residues using a radioactive UDP-glucose donor  
Includes
• T4 β-glucosyltransferase, 5 U/μL
• 10X Epi buffer
• 10X UDP-glucose
사양
효소T4 β-glucosyltransferase
함께 사용가능한 버퍼10X Epi Buffer
수량500 units
제품 유형T4 Beta Glucosyltransferase
Unit SizeEach
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