Thermo Scientific™

WELQut Protease (5 U/μL)

Thermo Scientific WELQut Protease is highly specific, recombinant serine protease of Staphylococcus aureus. It recognizes and precisely cleaves recombinant proteins자세히 알아보기

Thermo Scientific WELQut Protease is highly specific, recombinant serine protease of Staphylococcus aureus. It recognizes and precisely cleaves recombinant proteins containing an engineered recognition sequence† W- E- L- Q↓X (Trp, Glu, Leu, Gln, X can be any amino acid). The protease cleaves outside the recognition sequence without leaving additional amino acids bound to the target protein.

The WELQut Protease is active in a broad temperature (4 to 30°C) and pH (pH 6.5 to 9.0) range and does not require specific buffers.

In addition, this new protease has several procedural advantages - it is ideal for on-column proteolysis reactions and can be easily removed from reaction mixtures using its built-in His-tag.

Highlights

• Cleaves outside WELQ recognition sequence, without leaving additional amino acids bound to the target protein
• Highly specific to cognate recognition site, does not generate non-specific product bands, even after long incubation and using excess of protease
• Easy to remove from the reaction mixture using built-in His-tag
• Ideal for on-column proteolysis reactions

Applications

• Removal of N-terminal fusion tags from recombinant protein preparations.

Footnote

† This cleavage sequence is present in expression vector pLATE52 included into the Thermo Scientific aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WELQut) (#K1281) available from Thermo Scientific.

사양

제품라인WELQut

수량500 units

제품 유형Protease

Unit SizeEach