Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK5)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

2.5 µg per million cells

View 2 publications 2 publications

ELISA (ELISA)

0.5-2 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Neutralization (Neu)

0.004-0.015 µg/mL

-

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

TRFK5

Immunogen

Mouse T cell clone-derived partially purified recombinant mouse IL-5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609713

Product Specific Information

In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, recombinant mouse (rm) IL-5, rhIL-5, rmIL-3, or rmGM-CSF is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.