
Thermo Fisher Scientific Vesicular Acetylcholine Transporter (VAChT) Polyclonal Antibody
Rabbit polyclonal antibody against VAChT for detection in mouse and rat samples. Validated for Western blot and frozen tissue IHC. Lyophilized form, reconstitutable with DDW, stored at -20°C. High purity via antigen affinity chromatography, suitable fo...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:400 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)DAVRLREVQGKDGGE, corresponding to amino acid residues 495–509 of rat VAChT, cytoplasmic C-terminus |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: Add 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at −20°C.
Reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, aliquot and keep at −20°C.
Avoid repeated freeze/thaw cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
This gene belongs to the vesicular amine transporter family.
The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space.
Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase.
This gene is located within the first intron of the choline acetyltransferase gene.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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