Thermo Fisher Scientific ATG5 Polyclonal Antibody
ATG5 단백질을 인식하는 Thermo Fisher Scientific의 Sheep Polyclonal 항체. Western blot 및 IHC에 사용 가능하며, Human과 Mouse 반응성. 자가포식 및 세포사멸 연구에 적합. 동결건조 형태로 제공되며, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human autophagy protein 5 (ATG5, ASP, APG5, APG5L, hAPG5) conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: ATG5.
Target Information
ATG5 (Autophagy Related 5) is an essential protein for autophagy and may play an important role in apoptosis. It is involved in mitochondrial quality control, regulation of innate antiviral response, lymphocyte development, MHC II antigen presentation, and adipocyte differentiation.
Following conjugation to ATG12, the complex participates in autophagosome formation. ATG5 contributes to autophagic cell death via interaction with FADD. The ATG5–ATG12 conjugate forms a cup-shaped isolation membrane that detaches upon autophagosome completion.
APG5 expression occurs downstream of caspase activation during apoptosis. The ATG5–ATG12 conjugate also interacts with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and promoting viral replication.
Dysfunction of ATG5 is associated with spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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