Thermo Fisher Scientific PLK1 (Marker of Mitosis) Monoclonal Antibody (AZ44)
PLK1 단백질을 인식하는 Xenopus 유래 단클론 항체로, 세포 분열 마커 연구에 적합. Western blot 및 ELISA에 사용 가능하며, 단백질 A/G로 정제된 액상 형태. 인간 및 생쥐와 반응하지 않음. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Xenopus |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AZ44 |
| Immunogen | Recombinant full-length PLK1 Xenopus laevis protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
- Does not react with Human or Mouse.
Target Information
PLK1 is a serine/threonine kinase functioning in cell mitosis and is regarded as a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on specific motifs recognized by the POLO box domains.
PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, and WEE1.
It plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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