Thermo Fisher Scientific MGMT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (NP_0024032) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MGMT,uniProtId:P16455-1,ncbiNodeId:9606,antigenRange:1-207,antigenLength:207,antigenImageFileName:PA5-120050_MGMT_P16455-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120050_MGMT_P16455-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.29 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913622

Product Specific Information

Positive test controls include: Jurkat, Mouse liver, Mouse spleen. The target is usually found in the following locations: Nucleus.

Immunogen sequence: MLGQPAPLER FASRRPQVLA VRTVCDLVLG KMDKDCEMKR TTLDSPLGKL ELSGCEQGLH EIKLLGKGTS AADAVEVPAP AAVLGGPEPL MQCTAWLNAY FHQPEAIEEF PVPALHHPVF QQESFTRQVL WKLLKVVKFG EVISYQQLAA LAGNPKAARA VGGAMRGNPV PILIPCHRVV CSSGAVGNYS GGLAVKEWLL AHEGHRL

Target Information

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT`s ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.