Thermo Fisher Scientific MGMT Polyclonal Antibody
MGMT 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합합니다. 인간 및 생쥐 시료에 반응하며, 핵 내에 위치한 MGMT 단백질 검출에 유용합니다. 고순도 친화 크로마토그래피 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–207 of human MGMT (NP_0024032) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.29 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913622 |
Product Specific Information
- Positive test controls: Jurkat, Mouse liver, Mouse spleen
- Subcellular location: Nucleus
Immunogen sequence:
MLGQPAPLER FASRRPQVLA VRTVCDLVLG KMDKDCEMKR TTLDSPLGKL ELSGCEQGLH EIKLLGKGTS AADAVEVPAP AAVLGGPEPL MQCTAWLNAY FHQPEAIEEF PVPALHHPVF QQESFTRQVL WKLLKVVKFG EVISYQQLAA LAGNPKAARA VGGAMRGNPV PILIPCHRVV CSSGAVGNYS GGLAVKEWLL AHEGHRL
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. It protects normal cells from genotoxic and carcinogenic effects of DNA alkylation by removing alkyl groups from the O6 position of guanine. MGMT expression levels vary depending on cell type, tissue, species, and tumor characteristics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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