Thermo Fisher Scientific C4B Monoclonal Antibody (OTI1B2), TrueMAB

Applications

• Western Blot (WB): 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

This gene encodes the basic form of complement factor 4 (C4B), part of the classical activation pathway. The protein is expressed as a single chain precursor that is cleaved into alpha, beta, and gamma chains prior to secretion. The trimer facilitates interactions between antigen–antibody complexes and other complement components. The alpha chain can be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. The gene is located in the MHC class III region on chromosome 6 and exhibits haplotype variation, resulting in 1–3 copies per individual. Long and short forms exist due to the presence or absence of a 6.4 kb HERV-K retrovirus in intron 9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.