Thermo Fisher Scientific Gnb1 Polyclonal Antibody
Gnb1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot에 최적화되어 있으며 다양한 종에 반응. 고순도 항원 친화 크로마토그래피 정제, 안정적인 액상 형태로 제공. 연구용으로 시각 신호전달 관련 단백질 분석에 적합.
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Applications
- Western Blot (WB)
Tested Dilution: 1–3 µg/mL
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Fruit fly, Human, Mouse, Sheep, Rat |
| Published Species | Bovine, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic Peptide: R(8)QEA EQLKNQIRDARKAC(25) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325605 |
Product Specific Information
PA1-725 detects transducin beta (T beta) from sheep, bovine, mouse, and drosophila.
It has been successfully used in Western blot procedures, detecting an ~37 kDa protein representing Tr beta from retinal and optic nerve extracts of sheep and bovine, as well as mouse eye samples and drosophila.
The immunizing peptide corresponds to amino acid residues 8–25 from human Tr beta, completely conserved between human, amphibian, bovine, and rat.
PA1-725 immunizing peptide (Cat. # PEP-154) is available for use in neutralization and control experiments.
Target Information
Vision involves conversion of light into electrochemical signals processed by the retina and brain.
Rhodopsin absorbs light and becomes catalytically active, activating transducin—a GTP-binding protein. Activated transducin promotes cGMP hydrolysis by phosphodiesterase (PDE), reducing intracellular cGMP and causing ion channel closure and membrane hyperpolarization, leading to signal transmission.
Rhodopsin activity is terminated by phosphorylation followed by arrestin binding.
Rod transducin consists of Tr alpha, T beta, and T gamma; cone transducin consists of Tc alpha, T beta, and T gamma.
Differences in subunit composition contribute to varying light sensitivities between photoreceptive cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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