Thermo Fisher Scientific Grp94 Monoclonal Antibody (9G10)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Immunohistochemistry (IHC)

2-30 µg/mL

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

0.5-1 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

9G10

Immunogen

Purified Grp94 isolated from chicken oviducts. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GRP94,uniProtId:P14625-1,ncbiNodeId:9606,antigenRange:1-803,antigenLength:803,antigenImageFileName:11097-100UG_GRP94_P14625-1_Rat.svg,antigenImageFileNamePDP:11097-100UG_GRP94_P14625-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5-1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.