Thermo Fisher Scientific Vinculin (Marker of Age-related Macular Degeneration) Recombinant Rabbit Monoclonal Antibody (VCL/8533R)
Vinculin 단백질을 표적으로 하는 재조합 토끼 단일클론 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot 및 면역조직화학에 적합하며, 고순도 Protein A/G 정제 방식으로 제조되었습니다. 액상 형태, 4°C 보관, 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VCL/8533R |
| Immunogen | Recombinant fragment (around aa 174–322) of human Vinculin (VCL) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions is involved in the transmembrane assembly of adhesion plaques, where the beta subunit of integrin binds to talin, which then binds to vinculin. Vinculin interacts with alpha-actinin and possibly with itself, forming a key element in the linkage between the extracellular matrix and the cytoplasmic microfilament system. Mutations in this gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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