Thermo Fisher Scientific NeuroG3 Monoclonal Antibody (OTI5A8), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:150

-

Product Specifications

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI5A8

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 1-214of human Ngn3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Neurog 3 protein contains one basic helix-loop-helix (bHLH) domain.It is both necessary and sufficient to induce endocrine islet cell differentiation from embryonic pancreatic progenitors. Not only does itinitiates endocrine cell differentiation, italso promotes islet cell maturation and maintains islet function. It is reported that Neurogenin 3 can redirect the differentiation of bipotential secretory progenitors to endocrine rather than goblet cell fate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.