Thermo Fisher Scientific eIF4E Polyclonal Antibody
Rabbit polyclonal antibody targeting human eIF4E, validated for WB and IP applications. High specificity and affinity purified. Suitable for human and mouse samples. Supplied as liquid form, stored at 4°C. For research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 167 and 217 of human eukaryotic translation initiation factor 4E |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Rat, X. laevis, Bovine, and Rabbit
Target Information
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5′ ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide).
EIF4E is a eukaryotic translation initiation factor that directs ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists both as a free form and as part of the multiprotein complex EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA–ribosome binding step of protein synthesis.
Other subunits of EIF4F include EIF4A (50 kD, ATPase and RNA helicase activities) and EIF4G (220 kD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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