Thermo Fisher Scientific Amphiphysin Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 적합한 Amphiphysin 폴리클로날 항체. 인간 단백질에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS 버퍼와 글리세롤 함유. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human AMPH (Product #RP-92624) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789857 |
Product Specific Information
Immunogen sequence:
EKVIPSVVIE PASNHEEEGE NEITIGAEPK ETTEDAAPPG PTSETPELAT EQKPIQDPQP TPSAPAMGAA DQLASAREA
Target Information
Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein superfamily, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via their carboxy-terminal SH3 domain with dynamin, synaptojanin, and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminals. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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