Thermo Fisher Scientific DBT Polyclonal Antibody
DBT 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC에 적합합니다. 인간, 생쥐, 랫트 반응성이 검증되었으며 항원 친화 크로마토그래피로 정제되었습니다. PBS 기반 저장용액으로 구성되어 있으며 장기 보관 시 -20°C 보관 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 11–247 of Human DBT |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2547201 |
Product Specific Information
- Recommended positive controls: H1299, HepG2, mouse liver, rat liver
- Predicted reactivity: Mouse (95%), Rat (95%), Zebrafish (82%), Xenopus laevis (88%), Chicken (84%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%)
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of branched-chain amino acids isoleucine, leucine, and valine.
The BCKD complex is composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits.
This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2.
Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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