Thermo Fisher Scientific pJTI R4 Dest CMV N-EmGFP pA Vector

Thermo Fisher Scientific pJTI™ R4 Dest CMV N-EmGFP pA Vector

The pJTI™ R4 Dest CMV N-EmGFP pA vector enables expression of a gene of interest as an N-terminal Emerald Green Fluorescent Protein (EmGFP) fusion in a Jump-In™ parental cell line.
When used with a Jump-In™ parental cell line kit (e.g., Jump-In™ GripTite™ HEK293 cell line, Cat. No. A14150), it allows creation of isogeneic stable cell lines with less effort and time compared to traditional engineering methods.
The Gateway™ technology improves cloning efficiency for introducing the gene of interest (GOI) into the Gateway™-ready vector.
High retargeting efficiency via R4 sites enables use of isogenic pools for further experiments without clonal selection or facilitates easy selection of positive stable clones expressing the N-terminal EmGFP fusion.

The Jump-In™ Vectors and Parental Cell Lines Let You

• Develop stably engineered isogenic cell pools in about half the time of traditional methods
• Utilize isogenic expression from a defined genomic locus for comparative gene analysis
• Generate multiple cell lines in parallel with a simplified workflow
• Access Jump-In™ technology freely without complex licensing restrictions

Save Time with Rapid and Efficient Generation of Engineered Cell Lines

Using Gateway™ technology, you can generate an expression construct for retargeting with the pJTI R4 DEST CMV N-EmGFP pA destination vector.
In combination with a Jump-In™ parental cell line kit, the expression cassette is efficiently inserted into the genomic R4 site, enabling generation of functional cell pools in as little as two weeks without laborious clone isolation.
Clonal cell lines can also be generated faster due to the high percentage of positive clones.

Expand Your Experimental Capabilities

This vector and the Jump-In™ parental cell line provide an ideal solution for:

• Cells or assays where transient engineering is problematic
• Difficult-to-engineer cell lines
• Creating gene family, isoform, or ortholog panels

Gateway™ destination vectors accept large inserts, making them suitable for large or multi-unit proteins.

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