pJTI™ R4 Exp CMV EmGFP pA Vector

The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting자세히 알아보기

The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting reaction with a Jump-In™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector) included in the Jump-In™ parental kits, the EmGFP will be expressed and positive cells will fluoresce green.

Ensure the Success of Your Jump-In™ Retargeting Reactions
Successful retargeting of Jump-In™ parental cell lines like the the Jump-In™ GripTite™ HEK293 kit (A14150) is dependent on a variety of factors such as:

Transfection efficiency

• Cell confluency
• Antibiotic selection conditions
• Quality and concentration of DNA
• Retargeting vector to integrase vector ratio

We strongly recommend including a retargeting reaction with the pJTI™ R4 Exp CMV EmGFP pA vector in your Jump-In™ experiment along with negative controls (no plasmid DNA, no integrase vector) so you can easily visualize the results and optimize the retargeting conditions.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

사양

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

형식Liquid

프로모터CMV

제품 유형Mammalian Expression Vector

선택 제제(진핵)None

배송 조건Dry Ice

단백질 태그Untagged

클로닝 방법Gateway

수량100 μg

벡터Jump-In Vectors

제품라인Jump-In™

용도(애플리케이션)Stable Cell Line Development, Targeted Integration

Unit SizeEach