Thermo Fisher Scientific GADD45B Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human GADD45B. Recombinant protein control fragment (Product #RP-90634). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GADD45B,uniProtId:O75293-1,ncbiNodeId:9606,antigenRange:22-154,antigenLength:160,antigenImageFileName:PA5-56268_GADD45B_O75293-1_Rabbit.svg,antigenImageFileNamePDP:PA5-56268_GADD45B_O75293-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2641817

Product Specific Information

Immunogen sequence: AVEELLVAAQ RQDRLTVGVY ESAKLMNVDP DSVVLCLLAI DEEEEDDIAL QIHFTLIQSF CCDNDINIVR VSGMQRLAQL LGEPAETQGT TEARDLHCLL VTNPHTDAWK SHGLVEVASY CEESRGNNQW VPY

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.

Target Information

Cellcycle progression issubject to arrest at G1 and G2 checkpointsin response to DNA damage, presumablyto allow time for DNA repair prior to entryinto S andMphase, respectively. The p53 tumorsuppressor isrequired for one such G1 checkpoint and functionsto upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and inhibits entry ofcellsinto S phase. Thus, it has been suggested that GADD 45 mayserve as a link between the p53-dependentcellcycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45 beta and GADD 45 gamma, have been shown to be induced by environmentalstresses. GADD 45 beta and GADD 45 gamma are thought to induce p38/JNK activation via MEKK4 activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.