Thermo Fisher Scientific SQSTM1 Monoclonal Antibody (OTI4C8), TrueMAB
Human SQSTM1 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 사용 가능. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 NF-κB 신호전달 관련 단백질 분석에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C8 |
| Immunogen | Full-length human recombinant SQSTM1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The SQSTM1 gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the NF-κB signaling pathway.
It acts as a scaffolding/adaptor protein with TNF receptor-associated factor 6 to mediate NF-κB activation.
Alternatively spliced transcript variants encoding identical or distinct isoforms have been identified.
Mutations in SQSTM1 are associated with sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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