Thermo Fisher Scientific p53 Monoclonal Antibody (OTI2E4), TrueMAB

Applications

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation.
Upon stress, p53 activates transcription of genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory domain.

Phosphorylation at Ser15 is crucial for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells and acts as an anticancer agent. Elevated p53 levels are linked to aging, oxidative stress, and learning deficits due to impaired long-term potentiation (LTP).

In normal cells, p53 is expressed at low levels, but in transformed cell lines it is highly expressed and contributes to malignancy. Mutations in p53 disrupt DNA binding and tumor suppressor function. Alterations in the TP53 gene occur as somatic mutations in cancers and as germline mutations in Li-Fraumeni syndrome.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.