Thermo Fisher Scientific TRIM25 Polyclonal Antibody
TRIM25 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A로 정제된 1 mg/mL 액상 형태. 연구용으로만 사용 가능.
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Thermo Fisher Scientific TRIM25 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human TRIM25 (amino acids 531–630) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α.
Mice lacking this gene display an underdeveloped uterus and reduced estrogen responsiveness, indicating that TRIM25 is essential for estrogen-dependent proliferation.
TRIM25 expression increases in breast carcinoma, promoting tumor growth by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3σ.
Recent studies show that TRIM25 E3 ubiquitin ligase is crucial for the RIG-1 signaling pathway, mediating host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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