Thermo Fisher Scientific Nestin Polyclonal Antibody
Rat에서 반응하는 Goat Polyclonal Nestin 항체로, WB, IHC, ICC/IF에 사용 가능. 발달 중추신경계 및 줄기세포 마커 연구에 적합. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | - |
| Immunohistochemistry (IHC) | 5–15 µg/mL | 2 publications |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Human, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant rat Nestin Met544–Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609217 |
Product Specific Information
- In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin serves as a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, enhancing the therapeutic potential of human neural stem/progenitor cells in CNS disease treatment, and identifying neuroepithelial tumor cells.
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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