Thermo Fisher Scientific BRD4 Recombinant Rabbit Monoclonal Antibody (7L7M8)
BRD4 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA에 사용 가능. 인간 BRD4에 반응하며 높은 특이성과 재현성을 제공. 액상 형태로 보관 용이하며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7L7M8 |
| Immunogen | Synthetic peptide corresponding to amino acids 100–200 of human BRD4 (O60885) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911800 |
Product Specific Information
- Positive test controls: HeLa, Jurkat
- Target location: Chromosome, Nucleus
Immunogen sequence:
IIKTPMDMGT IKKRLENNYY WNAQECIQDF NTMFTNCYIY NKPGDDIVLM AEALEKLFLQ KINELPTEET EIMIVQAKGR GRGRKETGTA KPGVSTVPNT T
Target Information
BRD4 is a ubiquitously expressed nuclear phosphoprotein and a member of the BET family. It is the human homolog of mouse MCAP and contains two N-terminal bromodomains, a central ET-domain, and kinase-like motifs. BRD4 exists as two isoforms; the longer variant includes a proline- and glutamine-rich C-terminal region. It binds chromatin and regulates transcription and chromosomal dynamics during mitosis. BRD4 also interacts with proteins such as RFC and SPA-1, influencing cell growth and serving as a target for papillomavirus E2 protein.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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