Thermo Fisher Scientific GFI1B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

4-8 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human GFI1B if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GFI1B,uniProtId:Q5VTD9-1,ncbiNodeId:9606,antigenRange:1,antigenLength:330,antigenImageFileName:PA5-40328_GFI1B_Q5VTD9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40328_GFI1B_Q5VTD9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2607445

Product Specific Information

Peptide sequence: MPRSFLVKSK MAHTYHQPRV QEDEPLWPPA LTPVPRDQAP SNSPVLSTLF

Sequence homology: Cow: 83%; Dog: 100%; Goat: 83%; Horse: 100%; Human: 100%; Mouse: 83%; Pig: 93%; Rat: 82%

Target Information

GFI1b (zinc finger protein GFI-1b) is an essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. It is also a component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. GFI1b also acts as a transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. It also cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Mutations affecting the gene are associated with Bleeding disorder, platelet-type 17 (BDPLT17).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.