Thermo Fisher Scientific Dicer Polyclonal Antibody
Thermo Fisher Scientific의 Dicer Polyclonal Antibody는 인간, 생쥐, 랫트 반응성을 가지는 Rabbit IgG 항체입니다. Western blot, IHC, Flow Cytometry에 적합하며, E.coli 유래 Dicer 단백질을 면역원으로 사용했습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human Dicer recombinant protein (Position: M1–N195) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746270 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Dicer is a double-stranded RNA (dsRNA) endoribonuclease that plays a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves long dsRNAs and short hairpin pre-microRNAs (miRNA) into 21–23 nucleotide fragments with 3′ overhangs, producing short interfering RNAs (siRNA) and mature microRNAs. These guide the RNA-induced silencing complex (RISC) to complementary RNAs to degrade or inhibit translation.
RNA interference mediated by siRNAs contributes to transcript elimination from mobile and repetitive DNA elements and degradation of viral RNA, while the miRNA pathway regulates specific gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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