Thermo Fisher Scientific EYA2 Polyclonal Antibody
EYA2 단백질 인식용 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태, 4°C 보관. 인간 및 소 종에서 반응 예측.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and 150 of human eyes absent homolog 2 (EYA2, UniProt ID: O00167-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) recommended for FFPE tissue sections
- Based on 100% sequence identity, antibody predicted to react with Bovine
Target Information
EYA2 functions as both a protein phosphatase and transcriptional coactivator for SIX1, and likely also for SIX2, SIX4, and SIX5.
It acts as a tyrosine phosphatase that dephosphorylates Tyr-142 of histone H2AX (H2AXY142ph), promoting efficient DNA repair via recruitment of MDC1-containing complexes.
Phosphorylation of Tyr-142 on H2AX is a key marker distinguishing apoptotic versus repair responses to genotoxic stress.
EYA2 plays an important role in transcription regulation during organogenesis and contributes to hypaxial muscle development along with SIX1 and DACH2, functionally redundant with EYA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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