The AAVpro titration kit contains all of the reagents necessary for extraction of adeno-associated virus (AAV) particles from AAV-producing cells and real-time PCR analysis of the viral genomic sequence. This kit allows for more precise and rapid quantification of viral titer than conventional DNA blot and ELISA methods.
The AAVpro titration kit contains all of the reagents necessary for extraction of adeno-associated virus (AAV) particles from AAV-producing cells and real-time PCR analysis of the viral genomic sequence. This kit allows for more precise and rapid quantification of viral titer than conventional DNA blot and ELISA methods.
Regardless of the serotype (determined by the Cap gene used for packaging), the viral genome encapsulated within most commonly used recombinant AAV systems typically contain the ITRs (inverted terminal repeats) of AAV2. With this kit, amplification of the AAV2 ITRs is used to quantify titer and can, therefore, be used for determining the titer of any AAV serotype, as long as the ITRs are derived from AAV2. If you are unsure whether this kit will work for your vector, contact our technical services department with your vector sequence.
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