Thermo Fisher Scientific GRK2 Monoclonal Antibody (5D5)

Applications

Tested Dilution

Publications

Western Blot (WB)

3-5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5D5

Immunogen

Recombinant beta adrenergic receptor kinase 1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2225831

Product Specific Information

MA1-2013 detects the beta andrenergic receptor kinase 1 in human, mouse and rat samples.

MA1-2013 has been successfully used in Western blot, immunocytochemistry, immunofluoresence, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects a ~30 kDa protein representing the beta andrenergeic receptor kinase 1 in human HeLa cells. In immunocytochemistry procedures MA1-2013 recognizes the beta andrenergic receptor kinase 1 in HeLa, Hek-293 and 3T3 cells. Immunofluoresence in HeLa cells yeilds predominantely cytoplasmic membrane associated staining with some staining in the ruffles.

The MA1-2013 immunogen is recombinant beta adrenergic receptor kinase 1.

Target Information

ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.