Thermo Fisher Scientific Kaiso Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ZBTB33. Recombinant protein control fragment (Product #RP-91977). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Kaiso,uniProtId:Q86T24-1,ncbiNodeId:9606,antigenRange:113-228,antigenLength:672,antigenImageFileName:PA5-81890_Kaiso_Q86T24-1_Rabbit.svg,antigenImageFileNamePDP:PA5-81890_Kaiso_Q86T24-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2789051

Product Specific Information

Immunogen sequence: IAELGVPLSQ VKSISGTAQD GNTEPLPPDS GDKNLVIQKS KDEAQDNGAT IMPIITESFS LSAEDYEMKK IIVTDSDDDD DDVIFCSEIL PTKETLPSNN TVAQVQSNPG PVAISD

Target Information

This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-alpha 2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.