Thermo Fisher Scientific Rabbit anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Applications and Tested Dilution

Product Specifications

Product Specific Information

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2–8°C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins or human serum proteins.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in detection, sorting, or purification of their specified target. Secondary antibodies offer increased versatility, enabling use with various detection systems (e.g., HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Additional Formats

• Alexa Fluor 488 conjugate
• Alexa Fluor 594 conjugate
• Biotin conjugate
• Custom conjugation available upon request