Thermo Fisher Scientific Phospho-CHK2 (Thr432) Polyclonal Antibody
CHK2 단백질의 Thr432 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 사용 가능. 인간 및 마우스 시료에 반응. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CHEK2 (Accession O96017), corresponding to amino acid residues around phosphorylated Thr432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903109 |
Product Specific Information
This antibody detects endogenous levels of Chk2 only when phosphorylated at Thr432.
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are linked to various cancers. CHK2 is activated by DNA damage and phosphorylates multiple regulators of cell cycle control, including tumor suppressor proteins. It can also phosphorylate BRCA1, aiding in DNA damage repair. CHK2 is a putative tumor suppressor, with mutations associated with Li-Fraumeni syndrome and predisposition to sarcomas, breast, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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