Thermo Fisher Scientific PODXL Monoclonal Antibody (5F5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5F5

Immunogen

Purified recombinant fragment of human PODXL expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11153028

Product Specific Information

MA5-15846 targets PODXL in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15846 immunogen is purified recombinant fragment of human PODXL expressed in E. Coli. .

MA5-15846 detects PODXL which has a predicted molecular weight of approximately 49kDa.

Target Information

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.