Thermo Fisher Scientific PODXL Monoclonal Antibody (5F5)
인간 PODXL 단백질을 인식하는 마우스 단클론 항체로 ELISA, WB, IHC, FACS 등에 적합. 비결합형 액상 형태이며 4°C 또는 -20°C 보관. 인간 시료에 반응하며 예측 분자량은 약 49kDa.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F5 |
| Immunogen | Purified recombinant fragment of human PODXL expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153028 |
Product Specific Information
MA5-15846 targets PODXL in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human PODXL expressed in E. coli.
MA5-15846 detects PODXL with a predicted molecular weight of approximately 49 kDa.
Target Information
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane.
Other biological activities of the encoded protein include:
- Binding in a membrane protein complex with Na⁺/H⁺ exchanger regulatory factor to intracellular cytoskeletal elements
- Playing a role in hematopoietic cell differentiation
- Expression in vascular endothelial cells and binding to L-selectin
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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