Thermo Fisher Scientific Bu-1 Monoclonal Antibody (AV20), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/10^6 cells

-

Product Specifications

Species Reactivity

Chicken

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

AV20

Immunogen

Chicken peripheral blood lymphocytes and splenocytes

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Biotin
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with proprietary stabilizer

Contains

<0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745660

Target Information

Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated disulfide-linked homodimer. The monoclonal antibody AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. The monoclonal antibody AV20 does not cross react with turkey cells when analyzed by flow cytometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.