Thermo Fisher Scientific Villin Polyclonal Antibody
Villin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 적합합니다. 인간 및 생쥐 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 세포골격 연구 및 소화기계 암 마커 분석에 유용합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:20,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 7–286 of Human Villin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.36 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546554 |
Product Specific Information
- Recommended positive controls: A431, H1299, HepG2, mouse kidney
- Predicted reactivity: Mouse (89%), Rat (89%), Bovine (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Villin is part of the cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border.
It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner.
Villin serves as a specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in some Merkel cell tumors and adenocarcinomas of the lung, prostate, ovary, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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