Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright 600, eBioscience
마우스 Sca-1(Ly-6A/E) 항원을 인식하는 D7 클론의 Super Bright 600 결합 단일클론 항체로, 유세포분석용으로 최적화됨. 405 nm 레이저에서 여기되어 600 nm에서 방출하며, 줄기세포 및 면역세포 표지에 활용 가능. 4°C에서 암소 보관 권장.
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Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 38 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | D7 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2662382 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 645
- Request custom conjugation
Product Specific Information
The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 recognizes both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid populations, and peripheral T and B cells.
Sca-1 is expressed highly upon activation regardless of Ly-6 haplotype and serves as a major phenotypic marker for mouse hematopoietic progenitor/stem cell subsets.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Cell number should be optimized empirically (10⁵–10⁸ cells/test).
Fluorophore Details:
- Super Bright 600 is a tandem dye excited by 405 nm violet laser, emitting at 600 nm.
- Recommended filter: 610/20 bandpass.
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright conjugates to minimize non-specific polymer interactions.
- Light-sensitive; protect from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Stem Cell Antigen-1 (Sca-1) is a member of the Ly-6 family, expressed on multipotent hematopoietic stem cells (HSC) and used as a marker of HSC in mice. It is found in fetal liver, adult bone marrow, peripheral blood, and spleen, and may regulate B and T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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