Thermo Fisher Scientific Alpha-Synuclein Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400

-

Immunohistochemistry (Frozen) (IHC (F))

1:300

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat alpha-synuclein, Intracellular, C-terminus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Synuclein,uniProtId:P37377-1,ncbiNodeId:10114,antigenRange:97-110,antigenLength:140,antigenImageFileName:APZ-035-GP200UL_alpha_Synuclein_P37377-1_Guinea_pig.svg,antigenImageFileNamePDP:APZ-035-GP200UL_alpha_Synuclein_P37377-1_Guinea_pig_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinsons disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimers disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.