Thermo Fisher Scientific Estrogen Receptor alpha Polyclonal Antibody
에스트로겐 수용체 알파(ERα)를 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA 등에 사용 가능. 인간, 마우스, 랫트 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Estrogen Receptor alpha (amino acids 201–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen, undergoes a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA. This binding regulates gene transcription via recruitment of coactivator or corepressor proteins.
There are two major forms of the estrogen receptor, alpha (ESR1) and beta (ESR2). Alternative RNA splicing produces at least four known ERα isoforms:
- Isoform 1 (66 kDa)
- Isoform 2 (53 kDa)
- Isoform 3 (47 kDa)
- Isoform 4 (35 kDa)
Estrogen receptors are expressed in various tissues and are essential for sexual development, reproductive function, and bone homeostasis. They are implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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