Thermo Fisher Scientific NDUFS5 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human NDUFS5 protein. Validated for Western blot and immunohistochemistry. Affinity-purified with >95% purity. Supplied in PBS with 50% glycerol. Suitable for mitochondrial complex I research.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 51–106 of Human NDUFS5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2720991 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
NDUFS5 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5) is a subunit of mitochondrial complex I, located in the mitochondrial inner membrane. Complex I is the first and largest enzyme in the electron transport chain, responsible for oxidizing NADH and transferring electrons to ubiquinone, initiating oxidative phosphorylation. Defects in complex I subunits are associated with various myopathies and neuropathies. NDUFS5 is a 106 amino acid protein expressed in many human tissues, with higher expression in heart, skeletal muscle, liver, kidney, and fetal heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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