Thermo Fisher Scientific QSK Polyclonal Antibody
Human SIK3(QSK) 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태. 4°C에서 보관하며 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 350 and 400 of Human serine/threonine-protein kinase QSK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
SIK3 (QSK) is a serine/threonine-protein kinase belonging to the QIK subfamily.
Phosphorylation of SIK3 by LKB1 through 14-3-3 binding enhances its catalytic activity and leads to localization in punctate cytoplasmic structures.
Overexpression of SIK3 promotes G1/S cell cycle progression in ovarian cancer.
Mutations (H331L and A1103V) have been observed at significant frequency in breast cancer.
SIK3 acts as a novel tumor-associated antigen (TAA) and a positive regulator of mTOR signaling by triggering degradation of DEPTOR, an mTOR inhibitor.
It is involved in dynamic regulation of mTOR signaling during chondrocyte differentiation and negatively regulates the cAMP signaling pathway, possibly through CRTC2/TORC2 and CRTC3/TORC3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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