Thermo Fisher Scientific CD206 Monoclonal Antibody (MR5D3), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

MR5D3

Immunogen

Chimaeric CRD4-7-Fc protein.

Conjugate

PE PE PE

View additional formats

• Unconjugated
• Biotin
• FITC
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Protein G

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538350

Product Specific Information

CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilization. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a approximately 175 kDa type 1 membrane glycoprotein that is also known as CD206.

Target Information

CD206 (MSR, Mannose receptor, MRC1) is a 175 kDa transmembrane protein belonging to the group of pattern recognition receptors. CD206 is predominantly expressed in tissue macrophages, dendritic cells, a subpopulation of endothelial cells and sperm cells. CD206 is thought to play a role in the innate and adaptive immune response. CD206 is also expressed on microglia and mato cells of the brain but not astrocytes or neurons. CD206 also mediate the recognition and uptake of a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid b-protein (Abeta). While the normal role of CD206 is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and Amyloid beta deposition in Alzheimers disease (AD). CD206s gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. CD206 has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Other diseases associated with CD206 dysfunction include leprosy and Gaucher`s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.