Thermo Fisher Scientific PIP5K1A Polyclonal Antibody
PIP5K1A 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot과 IHC(파라핀 절편)에서 검증됨. 인산화 지질 신호전달 연구에 적합하며, 고순도 친화 크로마토그래피 정제 제품. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PIP5K1A (Accession Q99755), corresponding to amino acid residues S11–I61 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2854101 |
Product Specific Information
This antibody detects endogenous levels of total PIP5K1A.
Target Information
PIP5K1A (phosphatidylinositol-4-phosphate 5-kinase 1 alpha) is a lipid kinase that phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate.
It is required for Wnt3a-induced LRP6 phosphorylation at Ser1490 in mammalian cells.
Wnt3a stimulates phosphatidylinositol 4,5-bisphosphate formation via frizzled and disheveled, which directly interact with and activate PIP5K1A.
PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and early infection steps in permissive lymphocytes.
Overexpression of wild-type PI4P5K1A enhances HIV-1 Env-mediated PIP(2) production and viral replication in primary lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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