Thermo Fisher Scientific BOB-1 Recombinant Rabbit Monoclonal Antibody (2H5L2)
Recombinant rabbit monoclonal antibody for BOB-1 detection. Validated for ICC/IF and ChIP applications. High specificity and lot-to-lot consistency. Suitable for human samples and predicted to react with multiple species. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 2 µg/million cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2H5L2 |
| Immunogen | Protein corresponding to human BOB-1 [aa1–aa256] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2762372 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Bovine, and Sheep.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
BOB-1 is a transcriptional coactivator that specifically associates with OCT1 or OCT2. It enhances OCT1-mediated promoter activity and, to a lesser extent, that of OCT2. BOB-1 has no intrinsic DNA-binding activity and recognizes the POU domains of OCT1 and OCT2. It is essential for B-cell antigen response and germinal center formation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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