Thermo Fisher Scientific Nogo-A/B Polyclonal Antibody
Nogo-A/B 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간, 마우스, 랫 등 다양한 종에 반응합니다. WB, IHC, ICC, ELISA, IP 등 다중 응용에 적합하며, Protein G로 정제된 고순도 항체입니다. 뇌 조직에서 양성 대조군으로 사용 권장됩니다.
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Thermo Fisher Scientific Nogo-A/B Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Sheep, Rabbit, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to internal portion of human Nogo A (amino acids 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2254188 |
Product Specific Information
Suggested positive control: human, mouse, or rat brain tissue.
Target Information
Nogo-B is a myelin-associated splice variant of Nogo belonging to the reticulon family. It acts as a multidomain neurite growth inhibitory protein with regions that inhibit axonal regeneration in the adult CNS. Nogo-B and NGBR mediate chemotaxis in HUVECs and promote tube formation in 3D cultures. It is predominantly expressed in blood vessels, smooth muscle cells, and endothelial cells, contributing to vascular homeostasis and remodeling. Nogo-B is implicated in sporadic and familial amyotrophic lateral sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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