Thermo Fisher Scientific Phospho-PLK1 (Thr210) Polyclonal Antibody
PLK1 단백질의 Thr210 인산화를 인식하는 폴리클로날 항체. Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합. 인간, 생쥐, 랫드, 초파리 시료에서 반응. 세포 분열 및 암 연구에 유용. Thermo Fisher Scientific 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | - | 1 publication |
| ChIP assay (ChIP) | - | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Published species | Fruit fly |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a portion of mouse PLK1 phosphorylated at Thr210 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2539891 |
Product Specific Information
- Recognizes human, mouse (weak), rat (weak), and Drosophila (weak).
- Originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit.
- The antibody sold separately may require optimization for best performance.
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and is a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins bind and phosphorylate proteins already phosphorylated on motifs recognized by POLO box domains.
PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, and WEE1.
It plays a key role in centrosome function and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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